Also, everyone needs to post preprints too, so we did that too. First time for me. Anyway, Ali, Antonia, Marius, Stanley and I wrote this manuscript describing Ali and Antonia's work using catalytically inactivate Cas9 to block transcription factor binding at specific sites. This give a quick way to check the function of particular TF binding sites. It works because guide RNAs are longer than TF binding sites so flanking sequence can be used to generate specificity.

Check it out here! https://www.biorxiv.org/content/early/2018/03/15/282681

Posted
AuthorJan Skotheim

A massive thanks to all who attended and made it such a great meeting! From left to right: Amanda Amodeo (Princeton), Peter Pryciak (UMass), Gabriel Neurohr (MIT), Devon Chandler-Brown (Stanford), Fabian Rudolf (ETH), Mimi Xie (Stanford), Me, Matthew Swaffer (Stanford), Daniel Berenson (Stanford), Jon Turner (Stanford), Ben Topacio (Stanford), Ali Shariati (Stanford), Sirle and Mardo Koivomagi (Stanford), Aurora Alvarez-Buylla (Stanford), Kurt Schmoller (Munich), Rob de Bruin (UCL), Evgeny Zatulovskiy (Stanford), Bruce Futcher (SUNY).

Let the field be warned, we are going to get to the bottom on this question!

 

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Posted
AuthorJan Skotheim

Here we show how the adder phenomenon in budding yeast emerges, almost as an accident perhaps, from distinct regulation in G1 and S/G2/M phases of the cell cycle. This reconciles the adder hullabaloo with what we have previously been examining in terms of how growth triggers division at the G1/S transition.

http://www.cell.com/current-biology/abstract/S0960-9822(17)31024-2

Posted
AuthorJan Skotheim